NZYTaq II 2× Green Master Mix is a premixed ready-to-use solution containing NZYTaq II DNA polymerase (MB354), which belongs to a new generation of Taq-derived DNA polymerases optimized for standard PCR applications. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations and supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. MgCl₂ final concentration is 2.5 mM, allowing the implementation of a comprehensive range of PCR protocols. In addition, reactions assembled with NZYTaq II 2× Green Master Mix may be directly loaded onto agarose gels. The mix contains two dyes (blue and yellow) that allow monitoring the progress of the electrophoresis. NZYTaq II 2× Green Master Mix is not suitable when direct fluorescent or absorbance readings are required without prior purification of the amplified DNA from PCR. We recommend using the master mix version without dyes NZYTaq II 2× Colourless Master Mix (MB357) or purifying the PCR product using NZYGelpure (MB011) before performing such downstream protocols. NZYTaq II DNA polymerase lacks 3’→5’ exonuclease activity. Resulting PCR products have an A-overhang and are suitable for cloning with NZYtech’s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).