Supreme NZYTaq II DNA polymerase is an engineered version of NZYTaq II DNA polymerase displaying a hot-start-like PCR capacity. The enzyme activity at room temperature is limited, avoiding extension of non-specifically annealed primers or primer-dimers and thus providing higher specificity, sensitivity and yield during DNA amplification. The functional activity of the enzyme is restored during a short 5-minute incubation step at 95 °C. Once Supreme NZYTaq II DNA polymerase is activated, it displays high performance leading to high DNA yields in shorter extension times (15-30 s/kb) of DNA templates up to 6 kb. Supreme NZYTaq II DNA polymerase requires minimal optimization. In addition, the hot-start-like capacity of Supreme NZYTaq II DNA polymerase not only leads to higher PCR sensitivity but also allows a room-temperature reaction setup. Supreme NZYTaq II DNA polymerase lacks 3’→5’ exonuclease activity. Thus, resulting PCR products have an A-overhang and are suitable for cloning with NZYtech’s TA PCR cloning kits (MB053 or MB137).

Extension time: 15-30 sec/kb at 72 °C

3’→5′ activity: no

Product overhang: 3′-A

Fidelity (vs. Taq): 1x