NZYProof 2× Green Master Mix is a premixed ready-to-use solution containing NZYProof DNA polymerase, an enzyme that presents high fidelity and displays great performance in the majority of PCR applications. NZYProof DNA polymerase possesses 3’→5′ exonuclease proofreading capacity which enables the polymerase to amplify DNA with increased accuracy. The enzyme is highly efficient in the amplification of longer (≤10 kb) PCR products and site-directed mutagenesis. In addition, it is the recommended polymerase for routine cloning that requires precision. The master mix was optimized to allow high yields and enhanced sensitivity due the presence of stabilizer solution. It contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of DNA templates by PCR. Mg²⁺ final concentration is 1.5 mM, allowing the implementation of a variety of PCR protocols. In addition, reactions assembled with NZYProof 2× Green Master Mix may be directly loaded onto agarose gels. NZYProof 2× Green Master Mix contains two dyes (blue and yellow) that allow monitoring the progress of the electrophoresis. NZYProof DNA polymerase possesses 3’→5′ exonuclease proofreading activity. Resulting PCR products have blunt-ends and are suitable for cloning with NZYtech´s NZY-blunt PCR cloning kit (MB121).

Extension time: 60 sec/kb at 72 °C

Product overhang: blunt

3’→5′ activity: yes