NZYLong DNA polymerase is an engineered DNA polymerase designed to amplify long target DNA sequences, generally of 20 kb and beyond. A wide range of long PCR products can be generated using lambda DNA or human genomic DNA as starting template. The provided 10x reaction buffer protects DNA from depurination and nicking during long thermal cycling. NZYLong DNA polymerase offers a fivefold higher fidelity than conventional Taq DNA polymerases. The enzyme generates a mixture of A-overhangended (predominantly) and blunt-ended PCR products, being suitable for cloning with NZYtech’s TA PCR cloning kits (MB053 or MB137).