NZYLong 2× Colourless Master Mix is a premixed ready-to-use solution containing NZYLong DNA polymerase (MB003), an engineered DNA polymerase designed to amplify long target DNA sequences, generally of 20 kb and beyond. A wide range of long PCR products can be generated using lambda DNA or human genomic DNA as starting template. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of an extensive range of DNA templates. MgCl₂ final concentration is 2.5 mM, allowing the implementation of a variety of PCR protocols. For highly sensitive downstream applications it is recommended to purify the amplified PCR product using NZYGelpure (MB011) before usage in subsequent protocols. NZYLong DNA polymerase generates a mixture of A-overhang-ended (predominantly) and blunt-ended PCR products, being suitable for cloning with NZYtech’s TA PCR cloning kits (MB053 or MB137).

Extension time: 60 sec/kb at 68 °C

3’→5′ activity: no

Product overhang: mixed