Restriction enzymes are used to recognize a specific sequence of nucleotides and cut double stranded DNA into smaller fragments at a specific location. Each restriction enzyme recognises different DNA sequences. TaqI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the TaqI gene from Thermus aquaticus. TaqI activity is blocked by overlapping Dam methylation. The enzyme acts at 37 °C or alternatively at 65 °C.

Concentration: 10000 U/mL

Reaction Buffer: NZYtech provides TaqI with a specific buffer, NZYBuffer C. For double digestions please use 10x NZYBuffer U (MB110, not provided).

Activity in NZYtech Buffers:
NZYBuffer A: 80%
NZYBuffer B: 80%
NZYBuffer C: 100%
NZYBuffer U: 100%

Minimize exposure of enzyme to temperatures higher than -20 °C.

Incubation Temperature 37 °C/65 °C