Topoisomerase I (E. coli)

Topoisomerase I (E. coli) catalyses the relaxation of negatively supercoiled DNA as the enzyme has been implicated in the knotting and unknotting double-stranded DNA.
Specification Details
Product No MB43501
Unit Size 100 U
Storage Conditions -85 °C to -15 °C
Specification Details
Product No MB43501
Unit Size 100 U
Storage Conditions -85 °C to -15 °C
Shipping Conditions Dry Ice
Activity Supercoiled DNA relaxation
Product Category Other Modifying Enzymes
Reaction Temperature 37 °C
Heat Inactivation 65 °C
Concentration 5 U/μL

Topoisomerase I (E. coli) catalyses the relaxation of negatively supercoiled DNA as the enzyme has been implicated in the knotting and unknotting double-stranded DNA. In addition, the enzyme is involved in the linking of complementary rings of single-stranded DNA into double-stranded rings.
Applications:
– Relaxing negatively-supercoiled DNA

– Topoisomerase I (5 U/μL)
– Reaction Buffer (10x)