Exonuclease I (E. coli) is a typical DNA specific exonuclease that catalyses the removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction. The enzyme may be used to remove single-stranded primers in PCR reactions prior to Sanger DNA sequencing or SNP analysis nested PCR reactions. Exonuclease I (E. coli) may be used to generally remove linear single-stranded DNA, leaving behind double-stranded DNA in the sample.
Applications:
– Removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction
– Removal of single-stranded primers in PCR mixture prior to Sanger DNA sequencing
– Removal of linear single-stranded DNA, leaving behind double-stranded DNA in the sample