NZYtech’s Glycerol-free T4 DNA Ligase (50 U/µL) embodies an ultrapure recombinant enzyme, isolated from Escherichia coli, specialized in catalyzing the formation of phosphodiester bonds between abutting 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA structures.

This enzyme variant is meticulously engineered to furnish not only an elevated purity but also a high-concentration format devoid of glycerol in its storage buffer. Remarkably, this enzyme is furnished at a concentration that is ≈10x superior to standard T4 DNA Ligases. The unique storage buffer formulation endows it with compatibility for lyophilization, for both direct lyophilization processes and integration into complex master mixes intended for subsequent lyophilization.

The enzyme is optimized for applications that necessitate stringent quality and performance, such as high-complexity library cloning and Next-Generation Sequencing (NGS) library preparations. Glycerol-free T4 DNA Ligase joins both blunt and cohesive-end restriction fragments of duplex DNA or RNA and repairs single-strand nicks in duplex DNA. Specially designed to expedite your workflows, this enzyme is formulated to instantaneously ligate up to 250 ng of DNA at ambient laboratory conditions (20-25°C).

Remarkably, the enzyme can facilitate efficient ligation in a notably brisk timeframe of 20 seconds to 5 minutes at room temperature, streamlining and enhancing the efficiency of your molecular biology projects. The Glycerol-free T4 DNA Ligase is supplied with a meticulously optimized 4× Reaction Buffer to assure optimal enzymatic activity, streamline the process, and aid in achieving consistent and reliable results.