Nt.BbvCI is a Nicking Endonuclease purified from an Escherichia coli strain that carries the BbvCI fragment from Brevibacillus brevis (Bacillus brevis). The enzyme cleaves only one strand of DNA on a double-stranded DNA substrate. Nt.BbvCI activity is blocked by some combinations of overlapping CpG methylation. NZYtech provides Nt.BbvCI with a specific reaction buffer. NZYtech’s Nt.BbvCI Nicking Endonuclease is an enzyme that acts at 37 °C.