NZY Bst DNA polymerase belongs to a new generation of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus) DNA polymerases optimized for standard loop-mediated isothermal amplification (LAMP) applications. NZY Bst DNA polymerase displays 5´→3´ DNA polymerase activity together with a strong strand displacement activity, and thus lacks 5´→3´ exonuclease activity. The enzyme was engineered to produce robust LAMP amplifications in short running times, under minimal optimization conditions. NZY Bst DNA polymerase was engineered to provide high sensitivity, allowing the specific amplification of DNA fragments from low copy number templates. NZY Bst DNA polymerase is also suitable for nucleic acid amplification methods requiring strand displacement activity, such as Whole Genome Amplification (WGA) and Multiple Displacement Amplification (MDA).
Applications:
– Loop-mediated isothermal amplification (LAMP) applications
– Whole genome amplification (WGA)
– Multiple displacement amplification (MDA)