NZY Plant/Fungi gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from plant tissues and fungi samples. The method is spin column silica-based and requires no phenol or chloroform extraction. This kit uses two optimized lysis buffers systems based on the established CTAB and SDS methods. NZY Homogenization columns are included in this kit for conveniently clearing the crude lysates. The clear flow-through is mixed with Binding buffer PN for optimal binding of DNA to the silica membrane. Then, the DNA is selectively absorbed into the NZYSpin Plant Column and others impurities such as proteins and salts are removed during the washing steps.
The eluted genomic DNA has a A260/280 ratio between 1.7 and 1.9 what makes it ready to use in applications like sequencing, PCR, multiplex-PCR, genotyping and a wide range of other enzymatic manipulations.