NZY T7 High Yield RNA Synthesis kit was designed for the in vitro production of large amounts of RNA transcripts from any DNA template subjected to the control of a T7 promoter sequence. This kit was formulated to provide high yields of RNA transcripts. Yields of approximately 150-200 µg of RNA from 1 µg DNA template may be expected from a 2 hours reaction as described below. The resulting RNA transcripts present high integrity and the required quality for a variety of downstream applications, such as RNA splicing studies or RNA-protein interaction experiments. The in vitro transcription reaction is performed by an engineered version of phage T7 RNA polymerase. This enzyme displays a high specificity for the T7 promoter allowing an effective transcription of the DNA strand of interest from the promoter sequence. NZY T7 High Yield RNA Synthesis kit can be used to transcribe linearized plasmid DNA templates or DNA templates generated by PCR.

To maintain integrity of generated RNA transcripts, the T7 HY Enzyme Mix includes a Ribonuclease Inhibitor. This protein will effectively protect the synthesised RNA transcripts from degradation by contaminating RNases resulting from different sources, such as plasmid templates or others.