NZY T7 RNA Synthesis kit was designed for the in vitro production of RNA transcripts from any DNA template subjected to the control
of a T7 promoter sequence. In vitro transcription is performed by
an engineered version of phage T7 RNA polymerase. This enzyme
displays a high specificity for the T7 promoter allowing an effective transcription of the DNA strand of interest from the promoter sequence. NZY T7 RNA Synthesis kit can be used to transcribe linearized plasmid templates or DNA templates generated by PCR.

All kit components were optimized to carry out fast and efficient transcription of RNA. This kit is formulated to provide high yields of
RNA transcripts. Yields of approximately 50-80 µg of RNA from 0.5 µg DNA template may be expected from a 2 hours reaction as described below. The resulting RNA transcripts present high integrity and the required quality for a variety of downstream
applications, such as RNA splicing studies or RNA-protein interaction experiments.

To maintain integrity of generated RNA transcripts, the T7 Enzyme
Mix includes a Ribonuclease Inhibitor. This protein will effectively protect the synthesised RNA transcripts from degradation by
contaminating RNases resulting from different sources, such as plasmid templates or others