NZYMaxiprep

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NZYMaxiprep

NZYMaxiprep kit allows for rapid preparation of large-scale highly pure plasmid DNA from E. coli strains.
Specification Details
Product No –
Unit Size 2 columns, 5 x 2 columns
Storage Conditions 15 – 25 °C
Specification Details
Product No –
Unit Size 2 columns, 5 x 2 columns
Storage Conditions 15-25 °C
Shipping Conditions Room Temperature
Application In Vitro transcription, Cloning, PCR, Sequencing, Transformation
Starting Material E. coli culture
Scale Maxiprep
Features Manual
Format Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number
Product Category Column
Isolation Technology Plasmid DNA Purification, Silica membrane
Protocol time Approx. 90 min
NZYMaxiprep kit is designed for the rapid, large-scale preparation of highly pure plasmid DNA (typically 500 µg) from recombinant Escherichia coli strains. Plasmid DNA binds selectively to NZYtech columns charged with a silica-based anion-exchange resin. All contaminants, such as proteins, RNA, salts, nucleotides and oligos (<40-mer) are washed from the column. In the elution step, the positive charge of the resin is neutralized by a pH shift to slightly alkaline conditions and pure plasmid DNA is eluted in a high-salt elution buffer. To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 500 mL of E. coli cultures and double the volumes of Buffers M1, M2 and M3.

– Buffer M1
– Buffer M2
– Buffer M3
– Buffer MEQ
– Buffer MW
– Buffer ME
– RNase A
– NZYTech Plasmid Maxi Columns

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‪+49 1608703299‬

Neckarauer Waldweg 112
68199 Mannheim/ Germany
info@novomid.com

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