NZYMiniprep 96 well plate kit is designed for the manual or automated rapid, small-scale preparation of highly pure plasmid DNA from recombinant Escherichia coli strains. The method is based on the alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The plasmid DNA is selectively adsorbed in the silica gel-based NZYMiniprep 96 well plate and other impurities such as proteins, salts, nucleotides and oligos (<40-mer) are washed away. NZYMiniprep 96 well plate kit includes an additional washing buffer (AY) which is strongly recommended for the complete removal of high levels of endonucleases. NZYMiniprep 96 well plate kit allows simultaneous processing of up to 96 samples, typically in less than 45 min. Actual processing times may depend on the configuration of the liquid handling system used. The final concentration of the eluted DNA is 50–200 ng/μL (depending on the elution buffer volume and the bacterial culture). The kit is designed for use with vacuum manifolds or under centrifugation.