NZYProof DNA polymerase is a recombinant DNA polymerase that presents high fidelity and displays great performance in the majority of PCR applications. NZYProof DNA polymerase possesses 3´→5´ exonuclease proofreading capacity which enables the polymerase to amplify DNA with increased accuracy. The enzyme is highly efficient in the amplification of longer (≤10 kb) PCR products and site-directed mutagenesis. In addition, it the recommended polymerase for routine cloning that requires precision. The error rate of NZYProof DNA polymerase is similar to that of Pfu and Kod DNA polymerases and significantly lower than the error rate of Taq DNA polymerases. NZYProof DNA polymerase generates bluntended PCR products that are suitable for cloning with NZYtech’s NZY-blunt PCR cloning kit (MB121). NZYProof DNA Polymerase is supplied with a 10x Reaction Buffer, which contains Mg²⁺ at an optimal concentration for standard PCR, and a 5x Stabilizer Solution, which maximizes yields especially when using lower template concentrations.