NZYSpeedy Miniprep kit is designed for the ultra-rapid, small-scale preparation of highly pure plasmid DNA from recombinant Escherichia colistrains. NZYtech´s speedy miniprep procedure is based on the alkaline lysis of bacterial cells followed by adsorption of DNA onto a new specially treated silica membrane in the presence of high salt. The plasmid DNA is selectively adsorbed in the silica gel-based NZYtech plasmid spin column and other impurities such as proteins, salts, nucleotides and oligos (<40-mer) are washed away. The eluted DNA is suitable for applications like automated fluorescent sequencing, PCR and a wide range of other enzymatic manipulations. NZYSpeedy Miniprep kit includes a newly developed washing buffer (AS) which ensures complete removal of contaminants in a rapid washing step.