NZYSupreme Multiplex One-step RT-qPCR Probe Master Mix is an optimized and highly efficient reaction mixture developed for first strand cDNA synthesis and subsequent real-time PCR in a single tube. This offers great convenience and minimizes the risk of errors and contaminations. The master mix, formulated as a 2× reaction mixture, contains all components necessary for both cDNA synthesis and multiplex qPCR amplification (including enzymes, dNTPs, stabilizers and enhancers). Depending on the real-time thermal cycler used, up to 5-6 targets can be quantified simultaneously in the same well or tube. This master mix was engineered with a dual hot-start enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the NZYSupreme Multiplex One-step RT-qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes mixed in an optimized proportion specifically designed for the highest-performance. This master mix does not contain ROX and it was specifically developed for probe-detection technology.