NZYSupreme Multiplex qPCR Probe Master Mix (2x) is an optimized and highly efficient reaction mixture developed for real-time PCR. The master mix, formulated at a 2× concentration, contains all components necessary for multiplex qPCR amplification (including enzyme, dNTPs, stabilizers and enhancers). Depending on the real-time thermal cycler used, assays with up to 5-6 targets can be detected simultaneously in the same well/reaction. This master mix was engineered with an enzyme dual hot-start control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the NZYSupreme Multiplex qPCR Probe Master Mix (2x), including buffer chemistry and incorporation of highly robust engineered enzymes mixed in an optimized proportion specifically designed for the highest performance. This master mix was specifically developed for probe-detection technology.

Features:
– Convenient Multiplexing (up to 5-6 targets)
– Ultra-sensitive: detects low-copy number targets
– Dual hot-start mode
– Does not include a passive reference dye

Applications:
– Multiplex Real-time qPCR
– Developed for probe-detection technology