NZYTaq 2× GC-Enhancer Solution was specially developed to overcome difficulties in the polymerase chain reaction (PCR) amplification of GC-rich DNA templates using Taq DNA polymerase. The solution contains a proprietary formulation that has proven to be particularly useful when attempting to amplify highly GC-rich DNA sequences (GC% of 60-80%).

Successful amplification of highly GC-rich templates (>65%):

The addition of 2× GC-Enhancer Solution dramatically improves yields and specificity of problematic targets in PCR amplifications using NZYTaq DNA polymerase. To test the effect of this supplementation, six different human genomic DNA sequences with GC-contents ranging from 65 to 77 % were amplified, in parallel, using NZYTaq DNA polymerase or NZYTaq DNA polymerase plus the enhancer solution.NZYTaq 2x GC-Enhancer Solution_figure1The six human genomic DNA sequences (1-6) have a GC content of 77.2%, 66.4%, 68.7%, 72.9%, 71.6% and 65.7%, respectively. Results demonstrate a shift from non-specific products and smeared bands on agarose gel to the desired band in a wide range of GC-contents, when 2× GC-Enhancer Solution was used. Lane M: NZYDNA Ladder I.

Specifications:

Concentration: 2 ×
Compatible NZYtech DNA polymerases: NZYTaq II DNA polymerase (MB354); Supreme NZYTaq II DNA polymerase (MB355)
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped at 4 ºC to dry ice