NZYTaq II 2× Colourless Master Mix is a premixed ready-to-use solution containing NZYTaq II DNA polymerase (MB354), which belongs to a new generation of Taq-derived DNA polymerases optimized for standard PCR applications. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations and supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. MgCl₂ final concentration is 2.5 mM, allowing the implementation of a variety of PCR protocols. For highly sensitive downstream applications it is recommended to purify the amplified PCR product using NZYGelpure (MB011) before usage in subsequent protocols. Resulting PCR products have an A-overhang and are suitable for cloning with NZYtech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).

Extension time: 15-30 sec/kb at 72 °C
3’→5′ activity: no
Product overhang: 3′-A