NZYTaq II DNA polymerase belongs to a new generation of Taq-derived DNA polymerases that was engineered to produce high DNA yields in shorter PCR running times (15-30 s/kb extension) under minimal optimization conditions. NZYTaq II DNA polymerase supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. NZYTaq II is provided with 5× Gel Load Reaction Buffer allowing reactions to be loaded directly into gels without the extra adding of loading dye. This Gel Load Reaction Buffer is composed by a blue and yellow dye. The blue dye migrates at the same rate as a 3-5 kb DNA fragment in a 1% (w/v) agarose gel. The yellow dye migrates at a rate faster than primers (<50 bp) in a 1% (v/v) agarose gel. The 5× Gel Load Reaction Buffer is not suitable when direct fluorescent or absorbance readings are required without prior purification of the amplified DNA from PCR. NZYTaq II DNA polymerase lacks 3’→5’ exonuclease activity. Resulting PCR products have an A overhang and are suitable for cloning with NZYtech’s TA PCR cloning kits (MB053 or MB137).