Enzymatic cleanup of PCR products stands out for its precision, simplicity, speed, and efficiency compared to alternative methods like spin columns or nucleic acid purification beads.

The Speedy NZY Blue ExoSAP Mix features a potent combination of highly specific and pure recombinant Alkaline Phosphatase and Exonuclease activities. These two hydrolytic enzymes are tailored to process PCR products of varying sizes without any loss of sample integrity. Thus, the combinatory activity of this specialized mix eliminates unincorporated primers and nucleotides from PCR reactions, ensuring that resulting amplicons are efficiently processed during DNA sequencing, cloning, genotyping, or any other downstream applications.

Speedy NZY Blue ExoSAP Mix was optimized for commonly used PCR buffers, simplifying the cleanup process through the addition in the 1:6 ratio of Enzyme Mix to PCR product. The swift activity lasts only 2 minutes, followed by enzyme inactivation through a brief 1-minute heat exposure at 80°C.