Engineered to process an array of tissue materials, including blood and non-invasive hair samples, this kit promises a seamless, animal-friendly genotyping experience. The Direct Speedy Genotyping kit integrates a streamlined one-tube sample preparation with a swift PCR process, enabling rapid lysis of biological samples, including mouse genotyping tissues samples (tail clipping and ear punch), in under 5 minutes.

The kit is validated for hair sample genotyping, which executed with minimal animal discomfort and without necessitating anaesthesia harmoniously combines ethical and efficient genotyping. The Direct Speedy Genotyping kit includes a potent Speed Lysis Enzyme Mix, meticulously developed for rapid sample lysis at room temperature. Post-lysis, a brief centrifugation secures the DNA-laden supernatant.

Employing an aliquot of this lysate in tandem with the Speedy Supreme NZYTaq 2x Green Master Mix ensures lysis and PCR cycling times under one hour. In addition, the master mix contains a green dye for direct loading onto agarose gels, omitting the need for additional dye application. The Direct Speedy Genotyping kit also offers an alternative direct PCR protocol, facilitating PCR directly from undiluted, unpurified samples. The process produces ample template for numerous assays, compatible with a 96-well format, and assures sufficient template for a minimum of 20 genotyping PCR reactions. Finally, DNA fragments generated with the Direct Speedy Genotyping kit are 3’-dA-tailed and may be cloned into TA cloning vectors, or used for routine downstream analyses or applications, including restriction enzyme digestion, DNA cloning and sequencing.