Speedy NZYProof DNA polymerase belongs to a new generation of DNA polymerases displaying a fast polymerization rate. Only 5 seconds are required for the successful synthesis of a 1 kb size DNA fragment. Faster PCR can be further achieved by increasing the primers melting temperature, which increases PCR annealing temperature, thus allowing combining the annealing and extension PCR steps (see below). Speedy NZYProof DNA polymerase is supplied with an optimized 5× Reaction Buffer and a 50 mM MgCl₂ solution. The enzyme supports robust and reliable reactions while tolerating a wide range of templates. It is useful for fast and routine DNA amplifications that require fidelity, as well as for genotyping. PCR products generated by Speedy NZYProof DNA polymerase have blunt-ends and are suitable for cloning with NZYtech´s NZY-blunt PCR cloning kit (NZYtech, Cat. No. MB121).

Speed: 5 sec/kb

Product overhang: blunt