Supreme HC NZY First-Strand cDNA Synthesis Flexible Pack

Supreme HC NZY First-Strand cDNA Synthesis Flexible Pack

Provides all the required components to efficiently synthesize first-strand cDNA from an RNA template, offering flexible priming options. Patent pending submitted by NZYtech.
Specification Details
Product No MB49501
Unit Size 200 rxns
Storage Conditions -85 °C to -15 °C
Specification Details
Product No MB49501
Unit Size 200 rxns
Storage Conditions -85 °C to -15 °C
Shipping Conditions Dry Ice
Application cDNA synthesis, RT-PCR, RT-qPCR
Features Flexible primer selection, Fast protocol, High Accuracy and Processivity, Inhibitor resistant
Format Kit, Liquid
Product Category cDNA Synthesis
Reaction Temperature 60 °C
Heat Inactivation 85 °C
Protocol time Approx. 30 min.

Supreme HC NZY First-Strand cDNA Synthesis Flexible Pack is a versatile kit designed for efficient, fast and reliable first-strand cDNA synthesis. It offers a comprehensive solution, including:

  • High-Performance Reverse Transcriptase: Supreme NZY Reverse Transcriptase 1 kU/µL, known for its stability, accuracy, and ability to synthesize full-length cDNA;
  • Optimized Buffer: 10x Reaction Buffer specifically formulated for the reverse transcriptase, ensuring optimal enzyme performance;
  • RNase Inhibitor: Supreme NZY Ribonuclease Inhibitor is included to protect RNA from degradation;
  • dNTPs: A balanced mix of dNTPs for complete cDNA synthesis;
  • Primers: Both random hexamers and oligo(dT) 18 primers are provided for flexible priming options;
  • RNase H: For RNA degradation in cDNA-RNA hybrids which is recommended for downstream applications where RNA removal is necessary.

The produced cDNA can be used for various downstream applications, including real-time PCR, standard PCR, and microarrays.

Supreme HC NZY First-Strand cDNA Synthesis Flexible Pack offers a streamlined and efficient workflow for cDNA synthesis, providing researchers with a reliable and convenient tool for their molecular biology experiments.

Patent pending submitted by NZYtech.

    • Supreme NZY Reverse Transcriptase 1 kU/µL

 

    • Dilution Buffer for Supreme Reverse Transcriptase

 

    • Reaction Buffer 10x for Supreme NZY Reverse Transcriptase

 

    • Supreme NZY Ribonuclease Inhibitor

 

    • dNTP Mix (10mM each)

 

    • Oligo (dT)18 primer mix

 

    • Random hexamer mix

 

    • NZY RNase H (E. coli)

 

  • DEPC-treated H2O