T4 ssRNA Ligase catalyses the ATP-dependent formation of phosphodiester bonds between 5’-phosphate and 3’-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA. Note that the minimal substrate for T4 ssRNA Ligase is a nucleoside 3’,5’-biphosphate in intermolecular reaction and oligonucleotide of 8 bases in intramolecular reaction. The enzyme may be used for the ligation of ssRNA and DNA, 3´-termini of RNA labelling, synthesis of single-stranded oligoribo- and oligodeoxyribonucleotides and specific modifications of tRNAs.
Applications:
– Ligation of ssRNA and DNA
– 3´-end labelling of single strand RNA
– Synthesis of single-stranded oligoribonucleotides and oligodeoxyribonucleotides
– Specific modifications of tRNAs