T5 Exonuclease is a typical DNA nuclease that functions in the 5´ to 3´ direction and attacks both single and double-stranded DNA. The enzyme substrate is the 5´-termini of a DNA molecule in the context of single or double-stranded nucleic acids as well as gaps and nicks of linear or circular double-stranded DNA. However, T5 Exonuclease does not degrade supercoiled double-stranded DNA. Thus, the enzyme may be used to remove denatured DNA generated during the alkaline-lysis protocols for plasmid purification or linear and circular DNA from supercoiled plasmid preps. It may also be used to remove incomplete ligation products from ligated circular double-stranded DNA.
Applications:
– Degradation of linear ssDNA, dsDNA circular or nicked plasmid DNA
– No degradation of supercoiled plasmid DNA
– Removal of denatured DNA generated during alkaline-lysis plasmid purification procedures
– Removal of incomplete ligation products from ligated circular double-stranded DNA