T7 DNA Ligase is a typical DNA ligase that catalyses the formation of a phosphodiester bond between adjacent 5´ phosphate and 3´ hydroxyl groups of duplex DNA molecules. Cohesive end ligation and nick sealing are efficiently catalysed by T7 DNA Ligase, although the enzyme does not perform blunt end ligations. Thus, the enzyme discriminates between cohesive (active) and blunt (not active) fragment DNA ligations, as it only performs sticky end ligations. T7 DNA Ligase requires ATP for activity.
Applications:
– Cloning of DNA fragments generated by restriction enzymes digestion
– Nick-sealing in dsDNA
– Linear DNA circularization