Topoisomerase I (E. coli)

Topoisomerase I (E. coli) catalyses the relaxation of negatively supercoiled DNA as the enzyme has been implicated in the knotting and unknotting double-stranded DNA.
SpecificationDetails
Product NoMB43501
Unit Size100 U
Storage Conditions-85 °C to -15 °C
SpecificationDetails
Product NoMB43501
Unit Size100 U
Storage Conditions-85 °C to -15 °C
Shipping ConditionsDry Ice
ActivitySupercoiled DNA relaxation
Product CategoryOther Modifying Enzymes
Reaction Temperature37 °C
Heat Inactivation65 °C
Concentration5 U/μL

Topoisomerase I (E. coli) catalyses the relaxation of negatively supercoiled DNA as the enzyme has been implicated in the knotting and unknotting double-stranded DNA. In addition, the enzyme is involved in the linking of complementary rings of single-stranded DNA into double-stranded rings.
Applications:
– Relaxing negatively-supercoiled DNA

– Topoisomerase I (5 U/μL)
– Reaction Buffer (10x)